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ac p65 lys310  (Bioss)


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    Structured Review

    Bioss ac p65 lys310
    Ac P65 Lys310, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ac p65 lys310/product/Bioss
    Average 94 stars, based on 7 article reviews
    ac p65 lys310 - by Bioz Stars, 2026-02
    94/100 stars

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    Primary Antibodies Used in the Study

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: CD38 Deficiency Protects Mouse Retinal Ganglion Cells Through Activating the NAD+/Sirt1 Pathway in Ischemia-Reperfusion and Optic Nerve Crush Models

    doi: 10.1167/iovs.65.5.36

    Figure Lengend Snippet: Primary Antibodies Used in the Study

    Article Snippet: Ac-p65 , Cell Signaling , #3045S , Rabbit mAb , 1:1000 (WB).

    Techniques:

    Changes in Sirt1 and CD38 protein expression and assessment of the degree of deacetylation of downstream molecules in the ONC model. ( A ) The expression of Sirt1 protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. ( B ) The expression of CD38 protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. ( C ) The expression of Ac-p53 protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. ( D ) The expression of Ac-p65 protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. ( E ) The expression of IL-1β protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. ( F ) The expression of Caspase3 protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. WT, wild type; KO, knockout; data presented as: mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: CD38 Deficiency Protects Mouse Retinal Ganglion Cells Through Activating the NAD+/Sirt1 Pathway in Ischemia-Reperfusion and Optic Nerve Crush Models

    doi: 10.1167/iovs.65.5.36

    Figure Lengend Snippet: Changes in Sirt1 and CD38 protein expression and assessment of the degree of deacetylation of downstream molecules in the ONC model. ( A ) The expression of Sirt1 protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. ( B ) The expression of CD38 protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. ( C ) The expression of Ac-p53 protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. ( D ) The expression of Ac-p65 protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. ( E ) The expression of IL-1β protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. ( F ) The expression of Caspase3 protein in retina was detected by WB in the sham operation group and crush on day 3, day 7, and day 14. WT, wild type; KO, knockout; data presented as: mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Ac-p65 , Cell Signaling , #3045S , Rabbit mAb , 1:1000 (WB).

    Techniques: Expressing, Knock-Out

    To evaluate the effect of CD38 deletion on NAD+ content, acetylated protein content, and inflammatory and apoptotic protein expression in the retina in the optic nerve crush model. ( A ) In the crush day 7 model, the NAD+ kit was used to detect the changes of NAD + content in the retina of WT and KO mice. ( B ) In the crush day 7 model, the expression of Sirt1 protein in the retina of WT and KO mice was detected by WB. ( C ) In the crush day 7 model, the expression of Ac-p65 protein in the retina of WT and KO mice was detected by WB. ( D ) In the crush day 7 model, the expression of Ac-p53 protein in WT and KO retinas was detected by WB. (E) In the crush day 7 model, the expression of TNF-α protein in the retina of WT and KO mice was detected by WB. ( F ) In the crush day 7 model, WB was used to detect the changes of IL-1β protein expression in the retina of WT and KO mice. ( G ) In the crush day 3 model, WB was used to detect changes in Caspase3 protein expression in the retina of WT and KO mice. WT, wild type; KO, knockout; data presented as: mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: CD38 Deficiency Protects Mouse Retinal Ganglion Cells Through Activating the NAD+/Sirt1 Pathway in Ischemia-Reperfusion and Optic Nerve Crush Models

    doi: 10.1167/iovs.65.5.36

    Figure Lengend Snippet: To evaluate the effect of CD38 deletion on NAD+ content, acetylated protein content, and inflammatory and apoptotic protein expression in the retina in the optic nerve crush model. ( A ) In the crush day 7 model, the NAD+ kit was used to detect the changes of NAD + content in the retina of WT and KO mice. ( B ) In the crush day 7 model, the expression of Sirt1 protein in the retina of WT and KO mice was detected by WB. ( C ) In the crush day 7 model, the expression of Ac-p65 protein in the retina of WT and KO mice was detected by WB. ( D ) In the crush day 7 model, the expression of Ac-p53 protein in WT and KO retinas was detected by WB. (E) In the crush day 7 model, the expression of TNF-α protein in the retina of WT and KO mice was detected by WB. ( F ) In the crush day 7 model, WB was used to detect the changes of IL-1β protein expression in the retina of WT and KO mice. ( G ) In the crush day 3 model, WB was used to detect changes in Caspase3 protein expression in the retina of WT and KO mice. WT, wild type; KO, knockout; data presented as: mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Ac-p65 , Cell Signaling , #3045S , Rabbit mAb , 1:1000 (WB).

    Techniques: Expressing, Knock-Out

    To assess the effect of inhibition of Sirt1 on CD38 regulation of inflammatory responses and apoptosis in the ONC model. ( A ) NAD+ kit was used to detect the changes of NAD+ content in the retina of WT mice with/without EX527. ( B ) In the crush day 7 model, WB was used to detect changes in Sirt1 protein expression in the retina of KO mice with/without EX527. ( C ) In the crush day 7 model, the changes of GFAP protein expression in the retina of KO mice were detected by WB with/without EX527. ( D ) In the crush day 7 model, the expression of Ac-p65 protein was detected by WB in the retina of KO mice in the presence/absence of EX527. ( E ) In the crush day 7 model, WB was used to detect the changes of Ac-p53 protein expression in the retina of KO mice with/without EX527. ( F ) In the crush day 7 model, WB was used to detect the changes of TNF-α protein expression in the retina of KO mice with/without EX527. ( G ) In the crush day 3 model, WB was used to detect changes in Caspase3 protein expression in the retina of KO mice in the presence/absence of EX527. EX527, Sirt1 inhibitor; PBS, EX527 vehicle; KO, knockout; data presented as: mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: CD38 Deficiency Protects Mouse Retinal Ganglion Cells Through Activating the NAD+/Sirt1 Pathway in Ischemia-Reperfusion and Optic Nerve Crush Models

    doi: 10.1167/iovs.65.5.36

    Figure Lengend Snippet: To assess the effect of inhibition of Sirt1 on CD38 regulation of inflammatory responses and apoptosis in the ONC model. ( A ) NAD+ kit was used to detect the changes of NAD+ content in the retina of WT mice with/without EX527. ( B ) In the crush day 7 model, WB was used to detect changes in Sirt1 protein expression in the retina of KO mice with/without EX527. ( C ) In the crush day 7 model, the changes of GFAP protein expression in the retina of KO mice were detected by WB with/without EX527. ( D ) In the crush day 7 model, the expression of Ac-p65 protein was detected by WB in the retina of KO mice in the presence/absence of EX527. ( E ) In the crush day 7 model, WB was used to detect the changes of Ac-p53 protein expression in the retina of KO mice with/without EX527. ( F ) In the crush day 7 model, WB was used to detect the changes of TNF-α protein expression in the retina of KO mice with/without EX527. ( G ) In the crush day 3 model, WB was used to detect changes in Caspase3 protein expression in the retina of KO mice in the presence/absence of EX527. EX527, Sirt1 inhibitor; PBS, EX527 vehicle; KO, knockout; data presented as: mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Ac-p65 , Cell Signaling , #3045S , Rabbit mAb , 1:1000 (WB).

    Techniques: Inhibition, Expressing, Knock-Out